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Image Search Results
Journal: Frontiers in Immunology
Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging
doi: 10.3389/fimmu.2024.1383932
Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.
Article Snippet: LAG3 ,
Techniques: Imaging
Journal: Cancers
Article Title: Defining the Immune Checkpoint Landscape in Human Colorectal Cancer Highlights the Relevance of the TIGIT/CD155 Axis for Optimizing Immunotherapy.
doi: 10.3390/cancers14174261
Figure Lengend Snippet: Figure 1. Expression of IC by CD3+ TILs from CRC tumors according to the microsatellite status. For each figure, data were analyzed according to the MSS/MSI status, with MSS tumors shown in pale green and MSI tumors in dark blue. (A) Representative histograms showing the expression of PD-1, TIGIT, Tim-3, Lag3 and NKG2A on CD3+ TILs from an MSS patient (upper panels) or from an MSI patient (lower panels). Results were expressed as positive cells and median fluorescence intensity (MFI). Isotypic controls are overlaid in grey. (B) Frequency of PD-1+, TIGIT+, Tim-3+, Lag3+
Article Snippet: The following primary antibodies were used: CD3 (Agilent Technologies, polyclonal rabbit anti-human, RRID:AB_2335677), CD8 (Agilent Technologies, monoclonal mouse anti-human clone C8/144B, RRID:AB_2075537), PD-1 (Abcam, Cambridge, UK, monoclonal mouse antihuman clone NAT105, RRID:AB_881954), TIGIT (Cell Signaling Technology, Danvers, MA, USA; monoclonal rabbit anti-human clone ESY1W, RRID:AB_2922806), Tim-3 (Bio-Techne, Minneaoplis, MN, USA, polyclonal goat anti-human, RRID:AB_355235),
Techniques: Expressing
Journal: Cancers
Article Title: Defining the Immune Checkpoint Landscape in Human Colorectal Cancer Highlights the Relevance of the TIGIT/CD155 Axis for Optimizing Immunotherapy.
doi: 10.3390/cancers14174261
Figure Lengend Snippet: Figure 3. Identification of 14 CD8+ TIL clusters based on differential expression of ICs. (A) FlowSOM tree of CD8+ TILs from cohort 2 tumors (n = 20). The background coloring represents meta-clustering and the legends of the star plot and meta-clustering are shown on the right side. (B) Heatmap of the MFI of the PD-1, TIGIT, Tim-3, Lag3 and NKG2A markers expressed, or not expressed, by the 14 clusters identified in the CD8+ TIL population generated from cohort 2 tumors (n = 20). (C) Cell frequency in each CD4+ TIL cluster generated from cohort 2 tumors (n = 20). (D) Cell frequency in each CD4+ TIL cluster generated from cohort 2 and 3 tumors (n = 32) according to the MSS/MSI status (pale green and dark blue, respectively). The clusters of the two cohorts were merged and the cluster numbering from cohort 2 was retained; Mann–Whitney tests. (E) Principal component analysis of tumors from cohorts 2 and 3 based on cell frequencies in each generated CD8+ TIL cluster, with MSS tumors are in pale green triangles and MSI tumors in dark blue circles. Ellipses were drawn to highlight two new groups of colorectal tumors: the IChigh group (in dark blue) and the IClow group (in pale green).
Article Snippet: The following primary antibodies were used: CD3 (Agilent Technologies, polyclonal rabbit anti-human, RRID:AB_2335677), CD8 (Agilent Technologies, monoclonal mouse anti-human clone C8/144B, RRID:AB_2075537), PD-1 (Abcam, Cambridge, UK, monoclonal mouse antihuman clone NAT105, RRID:AB_881954), TIGIT (Cell Signaling Technology, Danvers, MA, USA; monoclonal rabbit anti-human clone ESY1W, RRID:AB_2922806), Tim-3 (Bio-Techne, Minneaoplis, MN, USA, polyclonal goat anti-human, RRID:AB_355235),
Techniques: Quantitative Proteomics, Generated, MANN-WHITNEY